Techno-functional properties of protein from protease-treated brewers’ spent grain (BSG) and investigation of antioxidant activity of extracted proteins and BSG residues

The study aimed to investigate the biological properties of protein fraction brewers’ spent grain (BSG) and its sediments as well techno-functional BSG (BSGP). was incubated with 0.5% protamex a combination flavourzyme, in addition control (incubation without protease). results showed that enzymatic treatment enhanced antioxidant activity BSGP. Compared sediment fraction, BSGPs had higher capacities than those sediments. current demonstrated FRAP value is aligned amount polyphenolic compounds, while BSGP responsible for ORAC ABTS capabilities. Enzyme on phenolic compounds treated proteases possessed oil-holding capacity, foaming lower emulsion capability. In conclusion, functionality bioactivity intensified allowing further valorization.